Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine
Identifieur interne : 005F03 ( Main/Exploration ); précédent : 005F02; suivant : 005F04Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine
Auteurs : Lin Tang [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Ede Qin [République populaire de Chine] ; Qingyu Zhu [République populaire de Chine] ; Man Yu [République populaire de Chine] ; Zhifen Ding [République populaire de Chine] ; Huiying Shi [République populaire de Chine] ; Xiaojie Cheng [République populaire de Chine] ; Caiping Wang [République populaire de Chine] ; Guohui Chang [République populaire de Chine] ; Shuangli Li ; Xumin Zhang [République populaire de Chine] ; Xishu Chen [République populaire de Chine] ; Jun Yu [République populaire de Chine] ; Ze Chen [République populaire de Chine]Source :
- Chinese Science Bulletin = Kexue Tongbao [ 1001-6538 ] ; 2003.
Abstract
A large quantity of SARS-CoV virus was proliferated in Vero cells, inactivated with β-propiolactone, then purified by Sepharose 4FF column chromatography to prepare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and electron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.
Url:
DOI: 10.1360/03wc464
PubMed: 32214708
PubMed Central: 7088751
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000353
- to stream Pmc, to step Curation: 000353
- to stream Pmc, to step Checkpoint: 001865
- to stream PubMed, to step Corpus: 003452
- to stream PubMed, to step Curation: 003452
- to stream PubMed, to step Checkpoint: 003155
- to stream Ncbi, to step Merge: 003539
- to stream Ncbi, to step Curation: 003539
- to stream Ncbi, to step Checkpoint: 003539
- to stream Main, to step Merge: 006389
- to stream Main, to step Curation: 005F03
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation, characterization and preliminary <italic>in vivo</italic> studies of inactivated SARS-CoV vaccine</title><author><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Qin, Ede" sort="Qin, Ede" uniqKey="Qin E" first="Ede" last="Qin">Ede Qin</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Man" sort="Yu, Man" uniqKey="Yu M" first="Man" last="Yu">Man Yu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Ding, Zhifen" sort="Ding, Zhifen" uniqKey="Ding Z" first="Zhifen" last="Ding">Zhifen Ding</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.419781.2</institution-id><institution-id institution-id-type="ISNI">0000000403885844</institution-id><institution>National Vaccine and Serum Institute,</institution></institution-wrap>100024 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100024 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Huiying" sort="Shi, Huiying" uniqKey="Shi H" first="Huiying" last="Shi">Huiying Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.419781.2</institution-id><institution-id institution-id-type="ISNI">0000000403885844</institution-id><institution>National Vaccine and Serum Institute,</institution></institution-wrap>100024 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100024 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Cheng, Xiaojie" sort="Cheng, Xiaojie" uniqKey="Cheng X" first="Xiaojie" last="Cheng">Xiaojie Cheng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Caiping" sort="Wang, Caiping" uniqKey="Wang C" first="Caiping" last="Wang">Caiping Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Li, Shuangli" sort="Li, Shuangli" uniqKey="Li S" first="Shuangli" last="Li">Shuangli Li</name></author><author><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Xishu" sort="Chen, Xishu" uniqKey="Chen X" first="Xishu" last="Chen">Xishu Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>430071 Wuhan, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>430071 Wuhan</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.411427.5</institution-id><institution-id institution-id-type="ISNI">0000000100893695</institution-id><institution>College of Life Science,</institution><institution>Hunan Normal University,</institution></institution-wrap>410081 Changsha, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>410081 Changsha</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32214708</idno><idno type="pmc">7088751</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088751</idno><idno type="RBID">PMC:7088751</idno><idno type="doi">10.1360/03wc464</idno><date when="2003">2003</date><idno type="wicri:Area/Pmc/Corpus">000353</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000353</idno><idno type="wicri:Area/Pmc/Curation">000353</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000353</idno><idno type="wicri:Area/Pmc/Checkpoint">001865</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001865</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:32214708</idno><idno type="wicri:Area/PubMed/Corpus">003452</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003452</idno><idno type="wicri:Area/PubMed/Curation">003452</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003452</idno><idno type="wicri:Area/PubMed/Checkpoint">003155</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003155</idno><idno type="wicri:Area/Ncbi/Merge">003539</idno><idno type="wicri:Area/Ncbi/Curation">003539</idno><idno type="wicri:Area/Ncbi/Checkpoint">003539</idno><idno type="wicri:doubleKey">1001-6538:2003:Tang L:preparation:characterization:and</idno><idno type="wicri:Area/Main/Merge">006389</idno><idno type="wicri:Area/Main/Curation">005F03</idno><idno type="wicri:Area/Main/Exploration">005F03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Preparation, characterization and preliminary <italic>in vivo</italic> studies of inactivated SARS-CoV vaccine</title><author><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Qin, Ede" sort="Qin, Ede" uniqKey="Qin E" first="Ede" last="Qin">Ede Qin</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Man" sort="Yu, Man" uniqKey="Yu M" first="Man" last="Yu">Man Yu</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Ding, Zhifen" sort="Ding, Zhifen" uniqKey="Ding Z" first="Zhifen" last="Ding">Zhifen Ding</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.419781.2</institution-id><institution-id institution-id-type="ISNI">0000000403885844</institution-id><institution>National Vaccine and Serum Institute,</institution></institution-wrap>100024 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100024 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Huiying" sort="Shi, Huiying" uniqKey="Shi H" first="Huiying" last="Shi">Huiying Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.419781.2</institution-id><institution-id institution-id-type="ISNI">0000000403885844</institution-id><institution>National Vaccine and Serum Institute,</institution></institution-wrap>100024 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100024 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Cheng, Xiaojie" sort="Cheng, Xiaojie" uniqKey="Cheng X" first="Xiaojie" last="Cheng">Xiaojie Cheng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Caiping" sort="Wang, Caiping" uniqKey="Wang C" first="Caiping" last="Wang">Caiping Wang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.410740.6</institution-id><institution-id institution-id-type="ISNI">0000 0004 1803 4911</institution-id><institution>Institute of Microbiology and Epidemiology,</institution><institution>Academy of Military Medicine,</institution></institution-wrap>100071 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>100071 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Li, Shuangli" sort="Li, Shuangli" uniqKey="Li S" first="Shuangli" last="Li">Shuangli Li</name></author><author><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Xishu" sort="Chen, Xishu" uniqKey="Chen X" first="Xishu" last="Chen">Xishu Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Beijing Genomics Institute,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>101300 Beijing, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>101300 Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff4"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences,</institution></institution-wrap>430071 Wuhan, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>430071 Wuhan</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff5"><institution-wrap><institution-id institution-id-type="GRID">grid.411427.5</institution-id><institution-id institution-id-type="ISNI">0000000100893695</institution-id><institution>College of Life Science,</institution><institution>Hunan Normal University,</institution></institution-wrap>410081 Changsha, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>410081 Changsha</wicri:regionArea></affiliation></author></analytic><series><title level="j">Chinese Science Bulletin = Kexue Tongbao</title><idno type="ISSN">1001-6538</idno><idno type="eISSN">1861-9541</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A large quantity of SARS-CoV virus was proliferated in Vero cells, inactivated with β-propiolactone, then purified by Sepharose 4FF column chromatography to prepare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and electron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P. A. Rota</name></author><author><name sortKey="Oberste, M S" uniqKey="Oberste M">M. S. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S. S. Monroe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ruef, C" uniqKey="Ruef C">C. Ruef</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dye, C" uniqKey="Dye C">C. Dye</name></author><author><name sortKey="Gay, N" uniqKey="Gay N">N. Gay</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Enserink, M" uniqKey="Enserink M">M. Enserink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pearson, H" uniqKey="Pearson H">H. Pearson</name></author><author><name sortKey="Clarke, T" uniqKey="Clarke T">T. Clarke</name></author><author><name sortKey="Abbott, A" uniqKey="Abbott A">A. Abbott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steinhoff, M C" uniqKey="Steinhoff M">M. C. Steinhoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lieu, T A" uniqKey="Lieu T">T. A. Lieu</name></author><author><name sortKey="Thompson, K M" uniqKey="Thompson K">K. M. Thompson</name></author><author><name sortKey="Prosser, L A" uniqKey="Prosser L">L. A. Prosser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rappuoli, R" uniqKey="Rappuoli R">R. Rappuoli</name></author><author><name sortKey="Miller, H I" uniqKey="Miller H">H. I. Miller</name></author><author><name sortKey="Falkow, S" uniqKey="Falkow S">S. Falkow</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E. D. Qin</name></author><author><name sortKey="Zhu, Q Y" uniqKey="Zhu Q">Q. Y. Zhu</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M. Yu</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Z" uniqKey="Chen Z">Z. Chen</name></author><author><name sortKey="Kadowaki, S" uniqKey="Kadowaki S">S. Kadowaki</name></author><author><name sortKey="Hagiwara, Y" uniqKey="Hagiwara Y">Y. Hagiwara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Budowsky, E I" uniqKey="Budowsky E">E. I. Budowsky</name></author><author><name sortKey="Friedman, E A" uniqKey="Friedman E">E. A. Friedman</name></author><author><name sortKey="Zheleznova, N V" uniqKey="Zheleznova N">N. V. Zheleznova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Budowsky, E I" uniqKey="Budowsky E">E. I. Budowsky</name></author><author><name sortKey="Smirnov, Y A" uniqKey="Smirnov Y">Y. A. Smirnov</name></author><author><name sortKey="Shenderovich, S F" uniqKey="Shenderovich S">S. F. Shenderovich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, A L" uniqKey="Smith A">A. L. Smith</name></author><author><name sortKey="De Souza, M S" uniqKey="De Souza M">M. S. De Souza</name></author><author><name sortKey="Finzi, D" uniqKey="Finzi D">D. Finzi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stephan, W" uniqKey="Stephan W">W. Stephan</name></author><author><name sortKey="Dichtelmuller, H" uniqKey="Dichtelmuller H">H. Dichtelmuller</name></author><author><name sortKey="Prince, A M" uniqKey="Prince A">A. M. Prince</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gupta, R K" uniqKey="Gupta R">R. K. Gupta</name></author><author><name sortKey="Relyveld, E H" uniqKey="Relyveld E">E. H. Relyveld</name></author><author><name sortKey="Lindblad, E B" uniqKey="Lindblad E">E. B. Lindblad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clements, C J" uniqKey="Clements C">C. J. Clements</name></author><author><name sortKey="Griffiths, E" uniqKey="Griffiths E">E. Griffiths</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Theodore, C E" uniqKey="Theodore C">C. E. Theodore</name></author><author><name sortKey="Martin, M" uniqKey="Martin M">M. Martin</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Li, Shuangli" sort="Li, Shuangli" uniqKey="Li S" first="Shuangli" last="Li">Shuangli Li</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tang, Lin" sort="Tang, Lin" uniqKey="Tang L" first="Lin" last="Tang">Lin Tang</name></noRegion><name sortKey="Chang, Guohui" sort="Chang, Guohui" uniqKey="Chang G" first="Guohui" last="Chang">Guohui Chang</name><name sortKey="Chen, Xishu" sort="Chen, Xishu" uniqKey="Chen X" first="Xishu" last="Chen">Xishu Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Chen, Ze" sort="Chen, Ze" uniqKey="Chen Z" first="Ze" last="Chen">Ze Chen</name><name sortKey="Cheng, Xiaojie" sort="Cheng, Xiaojie" uniqKey="Cheng X" first="Xiaojie" last="Cheng">Xiaojie Cheng</name><name sortKey="Ding, Zhifen" sort="Ding, Zhifen" uniqKey="Ding Z" first="Zhifen" last="Ding">Zhifen Ding</name><name sortKey="Qin, Ede" sort="Qin, Ede" uniqKey="Qin E" first="Ede" last="Qin">Ede Qin</name><name sortKey="Shi, Huiying" sort="Shi, Huiying" uniqKey="Shi H" first="Huiying" last="Shi">Huiying Shi</name><name sortKey="Wang, Caiping" sort="Wang, Caiping" uniqKey="Wang C" first="Caiping" last="Wang">Caiping Wang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Yu, Jun" sort="Yu, Jun" uniqKey="Yu J" first="Jun" last="Yu">Jun Yu</name><name sortKey="Yu, Man" sort="Yu, Man" uniqKey="Yu M" first="Man" last="Yu">Man Yu</name><name sortKey="Zhang, Xumin" sort="Zhang, Xumin" uniqKey="Zhang X" first="Xumin" last="Zhang">Xumin Zhang</name><name sortKey="Zhu, Qingyu" sort="Zhu, Qingyu" uniqKey="Zhu Q" first="Qingyu" last="Zhu">Qingyu Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005F03 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005F03 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:7088751
|texte= Preparation, characterization and preliminary in vivo studies of inactivated SARS-CoV vaccine
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32214708" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |